BRAKER2 is an extension of BRAKER1 which allows for fully automated training of the gene prediction tools GeneMark-EX and AUGUSTUS from RNA-Seq and/or protein homology information, and that integrates the extrinsic evidence from RNA-Seq and protein homology information into the prediction.

In contrast to other available methods that rely on protein homology information, BRAKER2 reaches high gene prediction accuracy even in the absence of the annotation of very closely related species and in the absence of RNA-Seq data.

BRAKER2 是 BRAKER1的扩展，它允许从 RNA-Seq 和/或蛋白质同源性信息全自动训练基因预测工具GeneMark-EX 和 AUGUSTUS，并将来自 RNA-Seq 和蛋白质同源性信息的外部证据集成到预测中。

与其他依赖蛋白质同源性信息的现有方法相比，BRAKER2 即使在没有非常密切相关物种的注释和没有 RNA-Seq 数据的情况下也能达到较高的基因预测精度。

  1. singularity build braker3.sif docker://teambraker/braker3:latest

参考

github 地址：https://github.com/Gaius-Augustus/BRAKER

公众号 | 生信媛 | 使用BRAKER2进行基因组注释（v2.1.5)

公众号 | 颤抖的小虫子 | 基因组结构注释软件—Braker教程