© Karobben

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Single Cell RNA-Seq Notes

Video materials: Human Cell Atlas: Day 1: HCA Latin America Single-Cell RNA-SEQ Data Analysis Workshop (Virtual)

Challenges

Barcodes errors

单细胞笔记 - 图1
© Dana Pe’er 2021

5000 cells in, but get 200,000 barcides identified.

Sbarcity

Sbarcity makes the noise is high and the protocols for bulk RNA-seq are not compectable for scRNA-Seq.

Demension

单细胞笔记 - 图2
© Dana Pe’er 2021

Reduce demention based on linear projection (PCA) is not compectable for hide dementional data

单细胞笔记 - 图3
© Dana Pe’er 2021

Batch effects

单细胞笔记 - 图4
© Dana Pe’er 2021

Path walk: psudotime

单细胞笔记 - 图5
单细胞笔记 - 图6
单细胞笔记 - 图7
单细胞笔记 - 图8
单细胞笔记 - 图9
© Dana Pe’er 2021

Nuclear RNS-Seq

Different between single cell RNA-Seq and single Nuclear RNA-Seq

单细胞笔记 - 图10
© Dana Pe’er 2021

Enjoy~

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