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samtools

Quick start

  1. samtools tview sorted.bam Trinity.fasta -p "ID:35" -d T > result
  2. samtools tview sorted.bam ../../2-Trinity/Trinity.fasta -p "comp0_c0_seq1:35" -d H > 123.html
  1. *.bam file *.fasta file -p posation, fasta name and star posation of the fasta :

Install

wget -c https://github.com/samtools/samtools/releases/download/1.12/samtools-1.12.tar.bz2
tar -xjf samtools-1.12.tar.bz2
cd samtools-1.12
ls
./configure
make & make install

Install through conda

conda install sra-tools

Sorting by bam files

samtools sort bwa.bam -o bwa.sorted.bam > bwa.sorted.bam

Index

samtools faidx genome.fna

Enjoy~

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