Basis

最全教程,我都看了:https://www.plob.org/category/single-cell

Pipeline

basic content(中文):https://zhuanlan.zhihu.com/p/484804392

environment(no seurat):https://github.com/theislab/single-cell-tutorial

Zhilong Jia:https://zhilongjia.github.io/scRNA/index.html

Transcriptome - 图1

Preprocessing

Cellrange

10X genomics:https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/what-is-cell-ranger

web:https://www.jianshu.com/p/5d8b87f4bd0e

Seurat(R-based)

Seurat4.0:https://satijalab.org/seurat/articles/pbmc3k_tutorial.html

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](https://satijalab.org/seurat/articles/pbmc3k_tutorial.html)